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Glutathione is a potent antioxidant crucial in preventing damage to cellular components caused by free radicals

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This enhances the transcription of antiapoptotic genes (e.g., CFLAR and TNFAIP3) and promotes the secretion of immunosuppressive cytokines (e.g., CCL2, CXCL1, and IL-10), recruiting ARG1TREM2-suppressive myeloid cells

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Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40